{"product_id":"mitochondrial-alterations-in-solid-carcinomas-relationship-between-tumorgenesis-of-human-kidney-carcinomas-and-mitochondrial-dna-mtdna-alterations-3836486407","title":"Mitochondrial Alterations in Solid Carcinomas: Relationship between Tumorgenesis of Human Kidney Carcinomas and Mitochondrial DNA (mtDNA) Alterations","description":"\u003cp\u003e\u003cstrong\u003eISBN:\u003c\/strong\u003e 3836486407\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eAuthor:\u003c\/strong\u003e Meierhofer, David\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eCondition:\u003c\/strong\u003e New\u003c\/p\u003e\u003cp\u003eAlterations of the respiratory chain and mutations of the mitochondrial DNA (mtDNA) have been extensively investigated in a wide variety of neoplasias. However, no obvious correlation of a certain type of tumor and mtDNA mutations has been reported so far. I compared both, enzymatic measurements of the respiratory chain and mtDNA mutations in renal carcinomas and renal oncocytomas. To enable a high throughput screen of mtDNA variations, I developed a DHPLC-based protocol for a rapid detection of unknown variations of the entire human mtDNA. The benign renal oncocytomas were in clear contrast to the malign renal carcinomas. The oncocytomas showed significantly increased Krebs cycle- and OXPHOS enzyme activities, except a total breakdown of the complex I enzyme activity. The mutation screening revealed frame shift mutations, encoding mitochondrial ND genes in 8 out of 12 oncocytomas. All these somatic frame shift mutations displayed a homoplasmy of 100%. This study shows for the first time a direct correlation of mtDNA mutations in a specific type of cancer, which leads to a total lack of complex I enzyme activity in the respiratory chain.\u003c\/p\u003e","brand":"Mia Karts","offers":[{"title":"Default Title","offer_id":51931751612704,"sku":"NEW3836486407","price":198.13,"currency_code":"USD","in_stock":true}],"url":"https:\/\/miakarts.com\/products\/mitochondrial-alterations-in-solid-carcinomas-relationship-between-tumorgenesis-of-human-kidney-carcinomas-and-mitochondrial-dna-mtdna-alterations-3836486407","provider":"Miakarts Books","version":"1.0","type":"link"}